immunoselect antifading mounting medium with dapi scr-038448  (Biotrend Chemicals)

Journal: Genes

Article Title: Olfactory Receptor OR2K2 Expression in Human Choroid Plexus as a Potential Marker in Early Sporadic Alzheimer’s Disease

doi: 10.3390/genes15030385

Figure Lengend Snippet: OR2K2 expression in CP. ( a , b ) Absolute measurement of OR ( a ) and TASR ( b ) gene transcription with selfie-dPCR. ( c – e ) OR2K2 relative protein expression in choroid plexus. Representative Western blot and relative quantification using C-terminal ( c ) and N-terminal ( d ) OR2K2 antibodies. Full immunoblots are presented in . * p < 0.05; ** p < 0.01; *** p < 0.001, One-way ANOVA followed by Bonferroni’s multiple comparison test. Ns, not significant. ( e ) Representative confocal microscopic images of choroid plexus epithelial cells demonstrating the expressions of OR2K2 (red) and nuclear stain DAPI (blue) in control subject (80-year-old male) and patients with AD (76-year-old male, AD Braak I, and 82-year-old male, AD Braak V). For the negative control, the choroid plexus section was incubated without primary antibody. Scale bar, 100 μm. In the lower panel, a magnification of the region is indicated in the white boxes in the upper panel. Scale bar, 20 μm. AD, Alzheimer’s disease.

Article Snippet: Finally, the slices were mounted with Immunoselect Antifading Mounting Medium with DAPI (SCR-038448, BioTrend, Köln, Germany).

Techniques: Expressing, Western Blot, Quantitative Proteomics, Comparison, Staining, Control, Negative Control, Incubation

Journal: Genes

Article Title: Olfactory Receptor OR2K2 Expression in Human Choroid Plexus as a Potential Marker in Early Sporadic Alzheimer’s Disease

doi: 10.3390/genes15030385

Figure Lengend Snippet: Colocalization of autophagic markers p62 and LC3 with OR2K2 in human CP. Immunohistochemistry of OR2K2 and p62 ( a ) and LC3 ( b ) was performed in CP sections. Representative confocal images of early (76-year-old male, AD Braak I) and advanced AD (82-year-old male, AD Braak V) cases compared with those from healthy donors (80-year-old male) are shown. Magnification insets from punctated boxes are presented on the right, with white arrows highlighting colocalization signals. OR2K2 (red), p62 (green), LC3 (green), and nuclear stain DAPI (blue). AD, Alzheimer’s disease. Scale bar, 100 μm.

Article Snippet: Finally, the slices were mounted with Immunoselect Antifading Mounting Medium with DAPI (SCR-038448, BioTrend, Köln, Germany).

Techniques: Immunohistochemistry, Staining

Journal: iScience

Article Title: Defects of renal tubular homeostasis and cystogenesis in the Pkhd1 knockout

doi: 10.1016/j.isci.2024.109487

Figure Lengend Snippet:

Article Snippet: Immunoselect Antifading Mounting Medium DAPI , Dianova/Biozol , Cat# SCR-038448.

Techniques: Recombinant, Knock-Out, Blocking Assay, Protease Inhibitor, Bicinchoninic Acid Protein Assay, SYBR Green Assay, In Situ, Software, Microscopy, Imaging